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Then, 3.5 l of the sample was applied onto 45 s glow-discharged quantifoil grids and vitrified in liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) at 100% humidity, 7 s blotting time and 10 s waiting time

Potential applications of amino acid metabolism regulation in the diagnosis and treatment of IPF Amino acids as emerging biomarkers in IPF Although traditional biomarkers such as KL-6, SP-D, MMP-7, TIMPs, and CCL18 remain commonly used in IPF diagnosis and monitoring [130], emerging evidence suggests that amino acid metabolic profiles may offer valuable diagnostic and prognostic insights